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The Campylobacter fetus S layer is not essential for initial interaction with HEp-2 cells
1Department of Biology, St. Francis Xavier University, Antigonish, NS, Canada. lgraham@stfx.ca
Canadian Journal of Microbiology
|June 2, 1998
Summary
The S layer of Campylobacter fetus subsp. venerealis does not appear essential for bacterial adherence to HEp-2 cells. Studies show S layer deficient strains attach to cells as efficiently as wild-type strains.
Area of Science:
- Microbiology
- Cell Biology
- Bacterial Pathogenesis
Background:
- Campylobacter fetus subsp. venerealis is a significant veterinary pathogen.
- The S layer is a surface protein layer found on many bacteria, often implicated in host-pathogen interactions.
- Understanding bacterial adherence mechanisms is crucial for controlling infections.
Purpose of the Study:
- To investigate the role of the S layer in the adherence of Campylobacter fetus subsp. venerealis to HEp-2 cells.
- To compare the adherence efficiency of S layer deficient strains with their wild-type counterparts.
Main Methods:
- In vitro adherence assays using HEp-2 cells and Campylobacter fetus strains (wild-type and S layer deficient isogenic mutants).
- Quantitation of bacterial adherence by light microscopy at various multiplicities of infection and time points.
- Comparison of adherence levels with Escherichia coli and Salmonella typhimurium.
Main Results:
- S layer deficient Campylobacter fetus strains did not show reduced adherence to HEp-2 cells compared to wild-type strains.
- Adherence levels were similar or even higher in S layer deficient strains.
- Campylobacter fetus adhered rapidly within the first 2 hours and did not replicate significantly post-adherence.
- Adherence was random, without localized patterns or changes in HEp-2 cell morphology.
Conclusions:
- The S layer is not essential for the initial interaction and adherence of Campylobacter fetus subsp. venerealis to HEp-2 cells.
- Other bacterial factors likely mediate the adherence of Campylobacter fetus to epithelial cells.
- Further research is needed to identify the specific adherence mechanisms involved.
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