Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Measuring transferrin receptor gene expression by NMR imaging

A Moore1, J P Basilion, E A Chiocca

  • 1Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown 02129, USA.

Biochimica Et Biophysica Acta
|June 2, 1998
PubMed
Summary

Researchers visualized human transferrin receptor (hTfR) expression and regulation using NMR imaging and a novel magnetic probe. This technique shows potential for imaging gene expression and transfer in vivo.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical diagnosis of symptomatic midfoot osteoarthritis: cross-sectional findings from the Clinical Assessment Study of the Foot.

Osteoarthritis and cartilage·2015
Same author

How are we using systemic drugs to treat psoriasis in children? An insight into current clinical U.K. practice.

The British journal of dermatology·2015
Same author

Weil's disease presenting as atypical pneumonia.

Acute medicine·2014
Same author

Development of haemophilic arthropathy of the ankle: results of a Delphi consensus survey on potential contributory factors.

Haemophilia : the official journal of the World Federation of Hemophilia·2014
Same author

A U.K. multicentre audit of the assessment and management of psoriasis in children.

The British journal of dermatology·2014
Same author

Abdominal organ bioengineering: current status and future perspectives.

Minerva chirurgica·2014

Area of Science:

  • Biophysics
  • Molecular Biology
  • Medical Imaging

Background:

  • The human transferrin receptor (hTfR) is a key target for drug delivery to tumors and across the blood-brain barrier.
  • Visualizing receptor expression and regulation is crucial for understanding therapeutic targeting and drug delivery efficacy.

Purpose of the Study:

  • To develop and validate a novel magnetic probe for visualizing hTfR expression and regulation using NMR imaging.
  • To demonstrate the feasibility of imaging receptor gene expression and regulation in vitro and in genetically engineered cell lines.

Main Methods:

  • Utilized a sterically protected, iron-containing magnetic probe designed to target the hTfR.
  • Employed NMR imaging to visualize the probe's interaction with hTfR in cells.
  • Used genetically engineered rat 9L gliosarcoma cell lines expressing different forms of hTfR.

Related Experiment Videos

Main Results:

  • The magnetic probe successfully entered cells via hTfR.
  • NMR imaging visualized hTfR expression and regulation in response to the probe.
  • The probe acted as an iron source but did not immediately downregulate hTfR within tested incubation times.
  • Successful visualization of hTfR expression and regulation was achieved in engineered cell lines.

Conclusions:

  • This study provides proof-of-principle for imaging receptor gene expression and regulation using a novel magnetic probe and NMR imaging.
  • The findings suggest the potential for in vivo imaging of gene transfer and therapeutic targeting via hTfR.