Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A quick method to extract DNA from paraffin-embedded tissues

C K Tseng1, N M Tsang, S C Kao

  • 1Department of Radiation Oncology, Chang Gung Memorial Hospital, Taoyuan, Taiwan, R.O.C.

Changgeng Yi Xue Za Zhi
|June 2, 1998
PubMed
Summary

This study presents a faster, more efficient DNA extraction method for paraffin-embedded tissues, improving polymerase chain reaction (PCR) amplification yields compared to conventional techniques.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measurement of the triple-differential dijet cross section in proton-proton collisions at <math> </math> and constraints on parton distribution functions.

The European physical journal. C, Particles and fields·2020
Same author

Measurements of the associated production of a Z boson and b jets in pp collisions at <math> </math>.

The European physical journal. C, Particles and fields·2020
Same author

Search for dark matter produced in association with heavy-flavor quark pairs in proton-proton collisions at <math> </math>.

The European physical journal. C, Particles and fields·2020
Same author

Study of dijet events with a large rapidity gap between the two leading jets in pp collisions at <math> </math> <math></math>.

The European physical journal. C, Particles and fields·2019
Same author

Measurement of associated Z + charm production in proton-proton collisions at <math> </math> <math></math>.

The European physical journal. C, Particles and fields·2019
Same author

Suppression of Excited ϒ States Relative to the Ground State in Pb-Pb Collisions at sqrt[s]_{NN}=5.02  TeV.

Physical review letters·2018

Area of Science:

  • Molecular Biology
  • Biochemistry

Background:

  • DNA extraction from paraffin-embedded tissues is crucial for retrospective studies using polymerase chain reaction (PCR).
  • Conventional DNA extraction methods are time-consuming and result in significant DNA yield loss.

Purpose of the Study:

  • To develop a more time-efficient and higher-yield DNA extraction method for paraffin-embedded tissues.
  • To compare the efficacy of the new method against conventional techniques.

Main Methods:

  • A novel method involving heating-melting-cooling paraffin removal, proteinase K digestion, and microconcentrator purification was employed.
  • DNA extracted using the new method was amplified for the p53 gene exon 8 via PCR.
  • Products were compared to those obtained using the conventional xylene-phenol-chloroform method.

Related Experiment Videos

Main Results:

  • The new DNA extraction method demonstrated superior performance in PCR amplification compared to the conventional approach.
  • Higher DNA recovery was observed with the proposed method.

Conclusions:

  • The developed method offers improved DNA recovery and is more time-efficient for processing paraffin-embedded tissues.
  • This technique is advantageous for molecular analysis requiring high-quality DNA yields.