Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

How platelet aggregation affects B16BL6 melanoma cell trafficking

C Koike1, M Watanabe, A Isoai

  • 1School of Pharmaceutical Sciences, University of Shizuoka, Japan.

FEBS Letters
|June 2, 1998
PubMed
Summary

Platelet aggregation minimally impacts tumor cell metastasis. Researchers found that inhibiting platelet aggregation only slightly reduced tumor cell accumulation in lungs, indicating it

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ER stress (PERK/eIF2alpha phosphorylation) mediates the polyglutamine-induced LC3 conversion, an essential step for autophagy formation.

Cell death and differentiation·2006
Same author

Beneficial action of candesartan cilexetil plus amlodipine or ACE inhibitors in chronic nondiabetic renal disease.

Journal of human hypertension·2004
Same author

Correlation between maximum bite force and craniofacial morphology of young adults in Indonesia.

Journal of oral rehabilitation·2003
Same author

Cavernous lymphangioma of the leg in children treated by the injection of OK-432 after resection.

The Journal of bone and joint surgery. British volume·2003
Same author

Cell kinetic study on histogenesis of Barrett's esophagus using rat reflux model.

Scandinavian journal of gastroenterology·2003
Same author

Coulomb dissociation of 8B and the low-energy cross section of the 7Be(p,gamma)8B solar fusion reaction.

Physical review letters·2003

Area of Science:

  • Oncology
  • Hematology
  • Cell Biology

Background:

  • Blood-borne metastasis involves tumor cells aggregating with platelets, adhering to endothelium, and invading basal membranes.
  • The precise role of platelet aggregation in metastatic tumor cell trafficking remains incompletely understood.

Purpose of the Study:

  • To investigate the contribution of platelet aggregation to metastatic tumor cell trafficking in vivo.
  • To evaluate the necessity of platelet aggregation for tumor cell arrest at target sites.

Main Methods:

  • Utilized highly invasive B16BL6 melanoma cells labeled with [2-18F]2-fluoro-2-deoxy-D-glucose.
  • Administered platelet aggregation inhibitors (cyclo(RSarDPhg)) and metastasis inhibitors (cyclo(GRGDSPA)) to mice.
  • Employed non-invasive positron emission tomography (PET) to track tumor cell trafficking in vivo.

Related Experiment Videos

Main Results:

  • Platelet aggregation inhibitor cyclo(RSarDPhg) did not inhibit overall metastasis.
  • Both cyclo(RSarDPhg) and cyclo(GRGDSPA) suppressed B16BL6 cell accumulation in the lungs by approximately 12%.
  • This suggests platelet aggregation plays a minor role in tumor cell trafficking and is not essential for target site arrest.

Conclusions:

  • Platelet aggregation contributes partially, but not critically, to metastatic tumor cell trafficking.
  • The study indicates that platelet aggregation is not an essential step for tumor cell arrest in target organs like the lungs.