Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Renal osteopontin expression in experimental urolithiasis

T Yagisawa1, P S Chandhoke, J Fan

  • 1Division of Urology, University of Colorado Health Sciences Center, Denver 80262, USA.

Journal of Endourology
|June 2, 1998
PubMed
Summary

Osteopontin (Opn) expression increases in rat kidneys with more severe kidney stone formation. This suggests osteopontin plays a role in combating calcium oxalate stone development in vivo.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

miR-216a rescues dexamethasone suppression of osteogenesis, promotes osteoblast differentiation and enhances bone formation, by regulating c-Cbl-mediated PI3K/AKT pathway.

Cell death and differentiation·2015
Same author

Distributions of topological observables in inclusive three- and four-jet events in pp collisions at [Formula: see text][Formula: see text].

The European physical journal. C, Particles and fields·2015
Same author

Evidence for Collective Multiparticle Correlations in p-Pb Collisions.

Physical review letters·2015
Same author

Constraints on parton distribution functions and extraction of the strong coupling constant from the inclusive jet cross section in pp collisions at [Formula: see text][Formula: see text].

The European physical journal. C, Particles and fields·2015
Same author

Search for a standard model Higgs boson produced in association with a top-quark pair and decaying to bottom quarks using a matrix element method.

The European physical journal. C, Particles and fields·2015
Same author

Search for dark matter, extra dimensions, and unparticles in monojet events in proton-proton collisions at [Formula: see text][Formula: see text].

The European physical journal. C, Particles and fields·2015

Area of Science:

  • Nephrology
  • Biochemistry
  • Urology

Background:

  • Osteopontin (Opn) is known to inhibit calcium oxalate crystallization in vitro.
  • The in vivo role of Opn in kidney stone formation remains unclear.

Purpose of the Study:

  • To investigate the renal expression of Opn in rats with varying degrees of experimental kidney stone formation.
  • To determine if Opn levels correlate with the severity of calcium oxalate deposition in the kidneys.

Main Methods:

  • Male Sprague-Dawley rats were divided into control, ethylene glycol (EG) treatment, and EG + ammonium chloride (NH4Cl) treatment groups.
  • Kidneys were analyzed for crystal deposition and Opn localization using immunohistochemistry.
  • Renal Opn expression was quantified in different kidney segments (proximal tubules, distal tubules, Henle's loops, collecting ducts).

Related Experiment Videos

Main Results:

  • Normal rats showed Opn staining in specific kidney tubules.
  • EG treatment led to increased Opn staining in tubules.
  • EG + NH4Cl treatment, causing significant crystal deposition, resulted in even greater Opn expression, including in collecting ducts and on the papillary surface, with Opn found within crystal deposits.

Conclusions:

  • Renal Opn expression intensifies and becomes more widespread with increasing severity of experimental urolithiasis.
  • These findings support the hypothesis that renal epithelial cells upregulate osteopontin production to counteract kidney stone development.