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In vitro bactericidal activities and morphologic changes in Escherichia coli and Bacteroides fragilis by
1Department of Obstetrics and Gynecology, Gifu University School of Medicine, Japan.
Abstract:
Polymicrobial infections with aerobes and anaerobes are common in pelvic inflammatory diseases. New parenteral cephalosporins have been widely used against those infections. We investigated in vitro morphologic changes and bactericidal activities on Escherichia coli and Bacteroides fragilis by cephalosporins; cefluprenam (CFLP), ceftazidime (CAZ) or cefotaxime (CTX). CFLP induced morphologic changes (filamentation) and bactericidal activities on E. coli and B. fragilis. Morphologic changes were observed by electron microscope. The filamentation induced by CFLP was time and concentration dependent. The bactericidal activity of CFLP against E. coli was almost equal to those of CAZ and CTX. The bactericidal activity of CTX against B. fragilis was superior to those of CFLP and CAZ. These results suggest that there are characteristic aspects in each cephalosporin.
Insights
New cephalosporins like cefluprenam show promise against polymicrobial infections common in pelvic inflammatory disease. Cefluprenam induced unique morphologic changes and bactericidal effects on E. coli and B. fragilis.
Area of Science:
- Microbiology
- Infectious Diseases
- Pharmacology
Background:
- Polymicrobial infections involving aerobes and anaerobes are prevalent in pelvic inflammatory diseases (PID).
- Parenteral cephalosporins are frequently employed to treat these challenging infections.
Purpose of the Study:
- To investigate the in vitro morphologic alterations and bactericidal efficacy of novel cephalosporins against key pathogens in PID.
- To compare the effects of cefluprenam (CFLP), ceftazidime (CAZ), and cefotaxime (CTX) on Escherichia coli and Bacteroides fragilis.
Main Methods:
- In vitro susceptibility testing and morphologic evaluation using electron microscopy.
- Time and concentration-dependent studies of cephalosporin-induced changes.
Main Results:
- Cefluprenam (CFLP) induced significant morphologic changes, specifically filamentation, in both E. coli and B. fragilis.
- CFLP demonstrated comparable bactericidal activity against E. coli to ceftazidime (CAZ) and cefotaxime (CTX).
- Cefotaxime (CTX) exhibited superior bactericidal activity against B. fragilis compared to CFLP and CAZ.
Conclusions:
- Cefluprenam exhibits distinct in vitro characteristics, including filamentation induction and broad-spectrum bactericidal activity.
- The study highlights characteristic differences in the in vitro activity profiles of various cephalosporins against common PID pathogens.
- These findings suggest potential for cefluprenam in treating polymicrobial infections, warranting further investigation.