[Comparative characteristics of the cytotoxic activity of antibrain and different antilymphocyte sera in vitro]

The cytotoxic test was used to assess the specificity of the action of antisera obtained by immunization of rabbits with the tissue of mouse brain, mouse intact lymphoid cells of various origin, and cells preliminarily filtered through a column of cotton. Anticerebral sera proved to react strictly specifically with the thymus-dependent population of lymphoid cells; antithymus sera behaved in analogous way both against the intact and against the filtered thymocytes. The action spectrum of the sera against the cells of the lymph nodes and the spleen depended on the methof obtained them. The sera against the intact cells of the lymph nodes reacted chiefly with the T-cells, killing 20% of the cells of bone marrow origin. The sera against the lymph node cells enriched by filtration were strictly specific; in comparison with the sera against crude cells, the sera against filtered splenic cells contained much more antibodies reacting with T-cells; admixture of antibodies against B cells was the same in the sera against crude and against filtered cells. Immunization of rabbits with mouse bone marrow cells permitted to obtain antiserum which gave a strictly specific reaction with B-cells after a corresponding absorption with thymocytes.