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Related Concept Videos

Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Non-nuclear Inheritance01:29

Non-nuclear Inheritance

Most DNA resides in the nucleus of a cell. However, some organelles in the cell cytoplasm⁠—such as chloroplasts and mitochondria⁠—also have their own DNA. These organelles replicate their DNA independently of the nuclear DNA of the cell in which they reside. Non-nuclear inheritance describes the inheritance of genes from structures other than the nucleus.
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

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The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
Export of Mitochondrial and Chloroplast Genes02:19

Export of Mitochondrial and Chloroplast Genes

A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred irrespective...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

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Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

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Published on: May 20, 2018

Linear mitochondrial genomes: 30 years down the line

J Nosek1, L Tomáska, H Fukuhara

  • 1Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.

Trends in Genetics : TIG
|June 5, 1998
PubMed
Summary

Many organisms possess linear mitochondrial DNA, challenging the circular DNA assumption. Studying these linear genomes offers insights into genome evolution, telomere biology, and potential drug targets for pathogens.

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Area of Science:

  • Mitochondrial genomics
  • Molecular biology
  • Evolutionary biology
  • Telomere biology

Background:

  • Historically, mitochondrial genomes were believed to be exclusively circular DNA molecules.
  • Recent discoveries reveal that a significant number of organisms harbor linear mitochondrial DNA.
  • This finding necessitates a re-evaluation of organelle genome structure and evolution.

Purpose of the Study:

  • To explore the implications of linear mitochondrial genomes for understanding organelle genome evolution.
  • To investigate the potential contribution of linear mitochondrial DNA studies to telomere maintenance and function.
  • To identify potential therapeutic targets by examining the replication mechanisms of linear mitochondrial DNA in human pathogens.

Main Methods:

  • Comparative genomics analysis of mitochondrial DNA structures across diverse organisms.
  • Bioinformatic approaches to study replication and maintenance mechanisms of linear mitochondrial DNA.
  • Comparative studies focusing on telomere-like structures in linear mitochondrial genomes.

Main Results:

  • Confirmation of widespread occurrence of linear mitochondrial DNA in various taxa.
  • Identification of unique sequence features and structural elements in linear mitochondrial genomes.
  • Preliminary data suggesting novel mechanisms for end-replication or protection in linear mitochondrial DNA.

Conclusions:

  • Linear mitochondrial DNA represents a significant departure from the established circular model, broadening our understanding of organelle genome diversity.
  • Research into linear mitochondrial genomes offers a unique window into evolutionary pathways and the fundamental biology of telomeres.
  • Targeting the replication of linear mitochondrial DNA in pathogens presents a promising strategy for developing novel antimicrobial therapies.