Assay for etoposide in human serum using solid-phase extraction and high-performance liquid chromatography with...

Area of Science:

• Analytical Chemistry
• Pharmacology
• Clinical Chemistry

Background:

• Etoposide is a crucial chemotherapeutic agent.
• Accurate quantification of etoposide in biological matrices is essential for therapeutic drug monitoring and clinical trials.
• Existing methods may lack the sensitivity or practicality for routine clinical use.

Purpose of the Study:

• To develop and validate a sensitive and reliable High-Performance Liquid Chromatography (HPLC) assay for the quantification of etoposide in human serum.
• To establish an assay suitable for clinical studies involving etoposide administration.

Main Methods:

• Development of a solid-phase extraction (SPE) method for serum sample preparation.
• Utilized High-Performance Liquid Chromatography (HPLC) with a C18 column for separation.
• Employed a fluorometric detector for etoposide and podophyllotoxin (internal standard) detection.

Main Results:

• The assay demonstrated a linear range of 0.5 to 20 microg/ml for etoposide.
• A low detection limit of 0.2 microg/ml was achieved.
• The method showed suitable retention times for etoposide (14 min) and podophyllotoxin (28 min).

Conclusions:

• The developed HPLC assay provides a reliable and sensitive method for etoposide quantification in human serum.
• This assay is well-suited for application in clinical studies and therapeutic drug monitoring of etoposide.