Comparative complement activation study of polypropylene hollow fibres of two different makes in static condition

The complement activation is one of the major problems encountered in the use of extracorporeal devices. The complement-activating potential of two polypropylene hollow fibres (used in membrane oxygenator) of different make and designated as F1 and F2 was tested with time (10, 30, and 180 minutes). The fibres were brought in contact with human blood under in vitro static condition for the comparison. A direct measurement of unadsorbed concentration of the complement protein, C3, present in the liquid phase of human blood before and after the contact with polymer was made using human C3 antisera. This gave a measure of C3 adsorption on the fibres with time and probably also gave an indirect measure of C3a in the blood. IgG was also estimated using antisera of human IgG. The total protein and albumin concentration were measured to obtain an overall adsorption profile of these protein on the fibre surfaces with respect to time. The results showed that C3 adsorption was taking place mainly through the alternative pathway over and above the classical one, being more in the case of F2 than F1. SEM studies revealed poor adhesion of platelets on both fibres, though some activated platelets were also seen with slight deformation at 10 minutes and a few with prominent pseudopodia formations at a later time period on the surface of both fibres. The total protein adsorption was faster, and the surface pores of the F1 were found masked at 10 minutes observation. Later, desorption occurred making the pores visible at 180 minutes. The F2 surface examination showed a continuous deposition of protein layers with time, thereby masking the pores at 180 minutes. The present experimental finding and assessment favoured the F1 as a marginally better candidate to be considered for oxygenator development.