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Salmonella mutagenicity of musk ambrette depends on both microsomal and bacterial enzyme activity
1Institute of Medical Microbiology and Hygiene, Faculty of Clinical Medicine Mannheim, University of Heidelberg, Mannheim, Germany.
Abstract:
Former studies revealed musk ambrette as a mutagen in Salmonella typhimurium TA 100 in the presence (+S9) but not in the absence (-S9) of an exogenous metabolizing system. To clarify the role of bacterial nitroreductases (NR) in the toxification of musk ambrette to mutagenic metabolites the compound was examined with the Salmonella/mammalian microsome assay using the NR deficient strain S.typhimurium TA 100 NR in the presence and absence of S9. Musk ambrette showed mutagenicity in Salmonella typhimurium TA 100 (+S9) but no mutagenicity in the NR deficient strain TA 100 NR (+S9). Additionally, no mutagenicity was detected in both TA 100 (-S9) and TA 100 NR (-S9). These results indicate the need for both mammalian microsomal enzymes and bacterial nitroreductases to cause the mutagenicity of musk ambrette.