Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Copper/zinc superoxide dismutase expression by different human osteosarcoma cell lines

B Grigolo1, G Lisignoli, S Toneguzzi

  • 1Laboratorio di Immunologia e Genetica, Istituto di Ricerca Codivilla Putti, I.O.R., Bologna, Italia.

Anticancer Research
|June 6, 1998
PubMed
Summary

This study investigated copper/zinc superoxide dismutase (Cu/Zn SOD) levels in human osteosarcoma cell lines. Findings suggest differentiation influences Cu/Zn SOD production and release, potentially impacting cancer progression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design, Development and Validation of a Knee Brace to Standardize the US Imaging Evaluation of Knee Osteoarthritis.

IEEE journal of translational engineering in health and medicine·2022
Same author

Development and validation of low-intensity pulsed ultrasound systems for highly controlled in vitro cell stimulation.

Ultrasonics·2021
Same author

Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care.

Stem cell reviews and reports·2021
Same author

Patient-specific meniscus prototype based on 3D bioprinting of human cell-laden scaffold.

Bone & joint research·2019
Same author

Comparison of growth factor and interleukin content of adult peripheral blood and cord blood serum eye drops for cornea and ocular surface diseases.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis·2018
Same author

Cultures of a human synovial cell line to evaluate platelet-rich plasma and hyaluronic acid effects.

Journal of tissue engineering and regenerative medicine·2018

Area of Science:

  • Oncology
  • Biochemistry
  • Cell Biology

Background:

  • Oxidative stress is linked to cancer development.
  • Antioxidant enzymes, like copper/zinc superoxide dismutase (Cu/Zn SOD), may counteract oxidative stress.
  • Cu/Zn SOD levels in tumors can differ from normal tissues, with varying reports in scientific literature.

Purpose of the Study:

  • To investigate the relationship between osteosarcoma cell differentiation and Cu/Zn SOD production and release.
  • To analyze Cu/Zn SOD levels in four human osteosarcoma cell lines (HOS, U-2 OS, MG63, Saos-2) and a normal stromal cell line.
  • To assess the effect of TNF alpha on Cu/Zn SOD release and intracellular levels in these cell lines.

Main Methods:

  • Culturing four human osteosarcoma cell lines (HOS, U-2 OS, MG63, Saos-2) and a normal human stromal cell line.

Related Experiment Videos

  • Measuring Cu/Zn SOD release into cell culture supernatant.
  • Quantifying intracellular Cu/Zn SOD levels.
  • Stimulating cells with TNF alpha to observe its effects.

    • Main Results:

    • HOS and U-2 OS cell lines showed higher Cu/Zn SOD release compared to MG63 and Saos-2, both in basal conditions and after TNF alpha treatment.
    • Intracellular Cu/Zn SOD levels were elevated in HOS and U-2 OS at 24 hours, decreasing over time, and increased following TNF alpha stimulation.
    • Differences in cell line behavior suggest a correlation between differentiation status and Cu/Zn SOD expression.

    Conclusions:

    • Osteosarcoma cell differentiation appears to influence copper/zinc superoxide dismutase (Cu/Zn SOD) production and release.
    • TNF alpha can modulate Cu/Zn SOD levels in osteosarcoma cells.
    • Further research into Cu/Zn SOD's role in osteosarcoma progression based on differentiation is warranted.