Effects of mechanical agitation on endothelial function of preserved corneas

Corneas from adult albino New Zealand rabbits were stored in either MK medium or moist chambers at 4 degrees C. for 18 to 24 hours. During that time, half the corneas from each group were agitated for 8 hours to simulate the motion that might occur during long-distance shipment. The corneas were then placed in specular microscopes and perfused for 4 hours with a modified Krebs-Ringer solution at 34 degrees C. Serial measurements of corneal thickness made during temperature reversal showed that agitation had no effect on endothelial viability of corneas stored in either solution. Electron microscopy of selected corneas confirmed this finding.