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Related Experiment Videos

Allergen challenge increases cell traffic between bone marrow and lung

L J Wood1, M D Inman, J A Denburg

  • 1Asthma Research Group, Department of Medicine, McMaster University, Hamilton, Ontario, Canada.

American Journal of Respiratory Cell and Molecular Biology
|June 25, 1998
PubMed
Summary

Allergen inhalation increases airway hyperresponsiveness and stimulates the proliferation and trafficking of inflammatory cells from bone marrow to lungs in dogs. This study used bromodeoxyuridine (BrdU) to track these proliferating cells during allergic responses.

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Area of Science:

  • Immunology
  • Respiratory Medicine
  • Cell Biology

Background:

  • Allergen inhalation in dogs with Ascaris suum can lead to increased inflammatory-cell progenitors in bone marrow (BM).
  • Airway hyperresponsiveness (AHR) is a key feature of allergic airway inflammation.
  • Understanding the trafficking of inflammatory cells and their progenitors is crucial for managing allergic diseases.

Purpose of the Study:

  • To investigate the effect of allergen challenge on the migration of inflammatory cells and their progenitors from the BM to the lungs.
  • To utilize bromodeoxyuridine (BrdU) as a marker for proliferating cells to track their movement.
  • To assess the development of AHR following allergen exposure.

Main Methods:

  • Dogs were divided into two groups, matched for AHR after screening allergen challenge.

Related Experiment Videos

  • One group inhaled allergen, the other inhaled diluent; both received BrdU injections.
  • Blood, BM aspirate, and bronchoalveolar lavage (BAL) samples were collected and analyzed for BrdU-positive cells using immunohistochemistry (IHC).

    • Main Results:

    • Allergen inhalation induced AHR at 24 hours post-challenge.
    • A significant increase in BrdU-positive cells was observed in blood (5.7% vs 2.5%) and BAL (3.2% vs 0.8%) after allergen challenge compared to diluent.
    • A trend towards increased BrdU-positive cells in BM (27.0% vs 18.9%) was noted, with a significant correlation between BAL neutrophils and BrdU-positive BAL cells.

    Conclusions:

    • Allergen inhalation stimulates an increase in proliferating cells, likely originating in the BM.
    • These proliferating cells traffic through circulation into the lungs in response to allergen exposure.
    • The findings highlight a mechanism of inflammatory cell recruitment in allergen-induced airway responses.