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Renal handling of [(14)C]mannose
The American Journal of Physiology
|July 1, 1976
Summary
This study investigated D-mannose uptake in rat kidney cortex slices, finding it a saturable, oxygen-dependent process. Extensive metabolism and accumulation of phosphorylated intermediates were observed, similar to D-glucose.
Area of Science:
- Biochemistry
- Renal Physiology
- Molecular Transport
Background:
- Understanding monosaccharide transport is crucial for renal function.
- D-mannose is a hexose sugar with implications in cellular metabolism.
Purpose of the Study:
- To characterize the uptake mechanism of D-mannose in adult rat kidney cortex.
- To investigate the metabolic fate of D-mannose within renal cells.
Main Methods:
- Incubation of adult rat kidney cortex slices with radiolabeled D-mannose.
- Analysis of uptake kinetics, oxygen dependency, and inhibition by various sugars.
- Chromatographic separation and identification of intracellular metabolites.
Main Results:
- D-mannose uptake demonstrated saturation kinetics and required oxygen.
- Uptake was inhibited by glucose, galactose, and phlorizin, suggesting shared transport pathways.
- Extensive metabolism occurred, producing CO2 and accumulating polar intermediates, including phosphorylated mannose.
- Intracellular mannose concentration did not exceed extracellular levels.
Conclusions:
- D-mannose transport in rat kidney cortex is an active, carrier-mediated process.
- The kidney metabolizes D-mannose, converting it into phosphorylated intermediates.
- The transport and metabolism pathways for D-mannose share similarities with those of D-glucose.