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Lactate production in isolated perfused rat lung
The American Journal of Physiology
|August 1, 1976
Summary
The isolated rat lung produces significant lactate from glucose aerobically. Hypoxia doubles lactate production, likely from amino acids, not anaerobic energy.
Area of Science:
- Biochemistry
- Physiology
- Respiratory System
Background:
- Lactate production in the lung is not fully understood.
- The metabolic fate of glucose in lung tissue requires further investigation.
Purpose of the Study:
- To investigate lactate production in the isolated perfused rat lung under aerobic and hypoxic conditions.
- To determine the metabolic sources of lactate synthesized by lung tissue.
Main Methods:
- Isolated perfused rat lung model.
- Use of [U-14C]glucose to trace metabolic pathways.
- Measurement of lactate production rates under varying oxygen conditions.
Main Results:
- Aerobic lactate production rate was 53 mumol/g dry wt/h, primarily from glucose.
- Hypoxia doubled lactate production, with only 60% derived from glucose.
- Lactate production remained elevated post-hypoxia, with increased glucose contribution.
Conclusions:
- Lung tissue exhibits high aerobic lactate production from glucose, exceeding glucose oxidation.
- Hypoxia stimulates lactate production, likely via amino acid catabolism, with minimal anaerobic energy generation.
- The metabolic effects of hypoxia on lung lactate production were not reversible within the experimental timeframe.