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Human endothelial cells regulate polymorphonuclear leukocyte degranulation

M K Topham1, H J Carveth, T M McIntyre

  • 1Nora Eccles Harrison Cardiovascular Research and Training Institute, Department of Medicine, University of Utah School of Medicine, Salt Lake City 84112-5000, USA.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|June 10, 1998
PubMed
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Human endothelial cells control neutrophil degranulation, releasing signaling factors like IL-8. This process is crucial for inflammatory responses and can impact vascular health.

Area of Science:

  • Immunology
  • Cell Biology
  • Vascular Biology

Background:

  • Neutrophil degranulation is vital for inflammatory responses.
  • Endothelial cells play a role in regulating neutrophil function.

Purpose of the Study:

  • To investigate how human endothelial cells regulate neutrophil degranulation.
  • To identify the signaling molecules involved in this process.

Main Methods:

  • Co-incubation of human neutrophils (PMN) with resting and activated human endothelial cells.
  • Stimulation of endothelial cells with cytokines like IL-1 and TNF-alpha.
  • Measurement of neutrophil degranulation markers (lactoferrin, elastase).
  • Use of blocking antibodies to identify signaling factors.

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Main Results:

  • Endothelial cells stimulated with IL-1 or TNF-alpha induced PMN degranulation, releasing lactoferrin and elastase.
  • PMN degranulation was dependent on incubation time, cytokine concentration, and PMN-endothelial cell contact.
  • Cytokine-stimulated endothelial cells produced newly synthesized degranulating factors, including IL-8.
  • An additional, unidentified degranulating factor was detected.

Conclusions:

  • Human endothelial cells actively regulate neutrophil degranulation through differential signaling.
  • Endothelial-derived factors influence neutrophil granular secretion, impacting inflammation.
  • Dysregulation of this process may contribute to vascular and tissue damage.