Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Distamycin-stabilized antiparallel-parallel combination (APC) DNA

A K Shchyolkina1, L E Minchenkova, E E Minyat

  • 1Engelhardt Institute of Molecular Biology, Russian Academy of Science, Moscow.

Journal of Biomolecular Structure & Dynamics
|June 10, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Crystallization and visible-near-infrared luminescence of Bi-doped gehlenite glass.

Royal Society open science·2019
Same author

Elevated α-synuclein caused by SNCA gene triplication impairs neuronal differentiation and maturation in Parkinson's patient-derived induced pluripotent stem cells.

Cell death & disease·2015
Same author

Alpha-Synuclein affects neurite morphology, autophagy, vesicle transport and axonal degeneration in CNS neurons.

Cell death & disease·2015
Same author

Visualization of lipid-receptor interactions on single cells by time-resolved imaging fluorescence microscopy.

Journal of fluorescence·2013
Same author

Targeting duplex DNA with chimeric α,β-triplex-forming oligonucleotides.

Nucleic acids research·2012
Same author

Effects of Oxidation Agents and Metal Ions on Binding of p53 to Supercoiled DNA.

Journal of biomolecular structure & dynamics·2012
Same journal

Decrypting the allosteric network: multi-scale dynamics of SARS-CoV-2 main protease inhibition by <i>Moringa oleifera</i> flavonoids.

Journal of biomolecular structure & dynamics·2026
Same journal

Anti-mycobacterial activity of phytocompounds from <i>Ricinus communis</i> L. - an integrated <i>in-vitro</i> and <i>in-silico</i> approach.

Journal of biomolecular structure & dynamics·2026
Same journal

Binding studies of the X-ray characterized [SnMe<sub>2</sub>Cl<sub>2</sub>(Me<sub>2</sub>phen)] complex with human serum albumin: experimental and molecular docking approaches.

Journal of biomolecular structure & dynamics·2026
Same journal

Computational design and experimental validation of peptide inhibitors to disrupt urease enzyme maturation in pathogenic bacteria <i>Proteus mirabilis</i>.

Journal of biomolecular structure & dynamics·2026
Same journal

Wavelet-domain multiway spectral separation of free drug, DNA, and drug-DNA complex profiles for quantitative binding analysis based on fractional occupancy (<i>θ</i>).

Journal of biomolecular structure & dynamics·2026
Same journal

Gene expression and microsecond scale conformational dynamics suggest potential regulatory mechanisms for the expanded subtilase family of <i>T. rubrum</i>.

Journal of biomolecular structure & dynamics·2026
See all related articles

Researchers explored Antiparallel-Parallel-Combination (APC) DNA structures, finding that the groove-binding ligand distamycin A stabilizes parallel-stranded segments. This DNA structure exhibits distinct groove geometries compared to conventional DNA, supporting its formation in solution.

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • Conventional DNA exists as an antiparallel-stranded (aps) double helix.
  • Parallel-stranded (ps) DNA segments are less common but can form within specific sequences.
  • Understanding DNA structural polymorphism is crucial for gene regulation and drug development.

Purpose of the Study:

  • To investigate the formation and stability of Antiparallel-Parallel-Combination (APC) DNA.
  • To characterize the interaction of the groove-binding ligand distamycin A (DstA) with APC DNA.
  • To elucidate the structural differences between APC DNA and conventional B-DNA.

Main Methods:

  • Synthesis of synthetic oligonucleotides designed to form APC DNA.
  • Stabilization of the parallel-stranded segment using distamycin A.

Related Experiment Videos

  • Analysis using circular dichroism and absorption spectroscopy, thermal denaturation, chemical modification, and molecular modeling.

    • Main Results:

    • Distamycin A binds to the parallel-stranded segment of APC DNA, with two drug molecules binding per complex.
    • APC DNA exhibits unique groove geometries (similar grooves, trans base pairs) compared to the distinct major and minor grooves of aps B-DNA (cis base pairs).
    • Differences in groove geometry significantly impact dye binding stoichiometry.

    Conclusions:

    • The study provides evidence supporting the existence and stability of APC DNA folding in solution.
    • Distamycin A effectively stabilizes the parallel-stranded segment within the APC DNA structure.
    • Structural variations in DNA helices influence ligand interactions and binding properties.