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The BRCA2 is a histone acetyltransferase

H Siddique1, J P Zou, V N Rao

  • 1Department of Human Genetics, Allegheny University of the Health Sciences, Philadelphia, Pennsylvania 19102, USA.

Oncogene
|June 10, 1998
PubMed
Summary

BRCA2 protein exhibits intrinsic histone acetyltransferase (HAT) activity, primarily acetylating histones H3 and H4. This newly identified HAT function suggests a role for BRCA2 in transcriptional regulation and tumor suppression.

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Area of Science:

  • Molecular Biology
  • Cancer Genetics
  • Biochemistry

Background:

  • Mutations in BRCA1 and BRCA2 tumor suppressor genes increase breast and ovarian cancer risk.
  • BRCA2 is implicated in transcriptional regulation and DNA repair.
  • Histone acetylation is linked to gene transcriptional activation.

Purpose of the Study:

  • To investigate the potential histone acetyltransferase (HAT) activity of BRCA2.
  • To determine if BRCA2 functions as a transcriptional regulator through HAT activity.

Main Methods:

  • Biochemical assays were used to test for HAT activity of BRCA2.
  • The specific region of BRCA2 responsible for HAT activity was mapped.
  • Acetylation targets of BRCA2 HAT activity were identified using free histones.

Main Results:

  • BRCA2 possesses intrinsic HAT activity.
  • The HAT activity of BRCA2 maps to its amino-terminal region.
  • BRCA2 primarily acetylates histones H3 and H4.

Conclusions:

  • BRCA2 exhibits intrinsic HAT activity, suggesting a novel mechanism for transcriptional regulation.
  • This HAT activity may contribute to BRCA2's role as a tumor suppressor.
  • Further research into BRCA2's HAT function could reveal new therapeutic targets for BRCA-associated cancers.

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