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Multiplex PCR for enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains from
S M Franck1, B T Bosworth, H W Moon
1Department of Pathology, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
Journal of Clinical Microbiology
|June 10, 1998
Abstract:
A multiplex PCR was developed to identify enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains by amplifying genes encoding K99 and F41 fimbriae, heat-stable enterotoxin a, intimin, and Shiga toxins 1 and 2. This multiplex PCR was specific and sensitive. It will be useful for identification of E. coli strains which cause diarrhea in calves.