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Stabilized, freeze-dried PCR mix for detection of mycobacteria
P R Klatser1, S Kuijper, C W van Ingen
1Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands. BO@mail.support.nl
Journal of Clinical Microbiology
|June 10, 1998
Abstract:
We report here the development of a freeze-drying procedure allowing stabilization at ambient temperature of preoptimized, premixed, and predispensed PCR mixes aimed at the detection of mycobacteria in clinical materials. The freeze-dried mixes retained activity at 4 degrees C and at 20 degrees C for 1 year and for 3 months at 37 degrees C, as judged by their performance with 50 and 500 fg of purified Mycobacterium bovis BCG target DNA.