Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rat liver genesine: a biochemical approach

L R Lerner1, M Gil, C R Krisman

  • 1Instituto de Investigaciones Bioquímicas Luis F. Leloir Fundación Campomar, Buenos Aires, Argentina.

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|June 10, 1998
PubMed
Summary

Genesine, a protein crucial for glycogen synthesis, was purified and studied. Its activity is enhanced by Mn2+, uses UDP-Glc as a substrate, and is inhibited by glucose and other molecules.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of a PET Insert for Trimodal Imaging: A Step Toward PET/MRI-Guided Focused Ultrasound.

IEEE transactions on radiation and plasma medical sciences·2026
Same author

[Annular lesions, diagnostic approach and treatment].

Semergen·2026
Same author

Distribution of microplastics present in a stream that receives discharge from wastewater treatment plants.

Environmental pollution (Barking, Essex : 1987)·2022
Same author

Remdesivir-Induced Nonimmediate Cutaneous Hypersensitivity Reaction.

Journal of investigational allergology & clinical immunology·2022
Same author

Sex and gender disparities in patients with advanced gastroesophageal adenocarcinoma: data from the AGAMENON-SEOM registry.

ESMO open·2022
Same author

What has changed during the state of emergency due to COVID-19 on an Academic Urology Department of a Tertiary Hospital in Portugal.

Actas urologicas espanolas·2020

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Glycogen biosynthesis is a critical metabolic pathway for glucose storage in animals.
  • Autoglucosylating proteins play a role in initiating or modifying polysaccharide synthesis.
  • Understanding the enzymes involved in glycogen synthesis provides insights into metabolic regulation.

Purpose of the Study:

  • To partially purify and characterize the biochemical properties of genesine from rat liver.
  • To identify factors affecting genesine activity, including metal ion activators and substrate preferences.
  • To investigate the mechanism of glucose transfer by genesine.

Main Methods:

  • Partial purification of genesine from rat liver tissue.
  • Enzyme activity assays to determine optimal conditions and substrate specificity.
  • Characterization of inhibitors affecting genesine activity.

Main Results:

  • Genesine activity was significantly activated by manganese ions (Mn2+).
  • Two distinct pH optima were observed for genesine activity.
  • Uridine diphosphate glucose (UDP-Glc) was the preferred glucosyl donor, though UDP-xylose also showed activity.
  • Multiple glucose units were transferred to the protein or existing glucan chains.
  • Glucose, maltose, xylose, and UDP were identified as inhibitors of genesine.

Conclusions:

  • Genesine is an autoglucosylating protein involved in glycogen biosynthesis with specific biochemical requirements.
  • The enzyme exhibits unique properties, including Mn2+ activation and dual pH optima.
  • Its substrate specificity and inhibitory profile offer insights into its role in glycogen metabolism.

Related Experiment Videos