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Platelet endothelial cell adhesion molecule-1 expression during mouse postimplantation development
G Calabrese1, M Fernandez de Recondo, E Recondo
1Cátedra de Biología Celular e Histología, Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Abstract:
With the aim to analyze the ontogeny of the mouse endothelium, monoclonal antibody (mAb) MEC 13.3 was used for the immunohistochemical staining of frozen sections of different stages of mouse embryo development. This mAb specifically recognizes membrane reinforcement of endothelial cells (EC) from mouse blood vessels, indicating the expression of a molecule related to the murine form of PECAM-1/CD31. The present study reports the expression of the murine PECAM-1/CD31 antigen, observed with the peroxidase-antiperoxidase technique, in a single cell type, with a typical non-differentiated morphology at an early stage of mouse postimplantation embryos. A progressive increase in the number of this cell type was observed in the early stages of murine development, but few were detected at mature stages. On the other hand, EC in days 9.5, 14.5 and 19.5 postcoitum embryos were also recognized by the same mAb MEC 13.3 allowing the recognition of a cell type related directly or indirectly to vascular network development.
Insights
This study analyzed mouse endothelium development using monoclonal antibody MEC 13.3. It identified the early expression of a PECAM-1/CD31-related antigen in non-differentiated endothelial cells during mouse embryogenesis.
Area of Science:
- Developmental Biology
- Immunohistochemistry
- Vascular Biology
Background:
- Understanding endothelium ontogeny is crucial for developmental biology.
- Monoclonal antibody (mAb) MEC 13.3 recognizes a molecule related to mouse PECAM-1/CD31.
- Endothelial cells (EC) form the blood vessel network.
Purpose of the Study:
- To analyze the ontogeny of mouse endothelium.
- To investigate the expression of PECAM-1/CD31 during mouse embryonic development.
- To characterize early endothelial cell markers.
Main Methods:
- Immunohistochemical staining of frozen sections from different stages of mouse embryo development.
- Utilized monoclonal antibody (mAb) MEC 13.3.
- Employed the peroxidase-antiperoxidase technique.
Main Results:
- mAb MEC 13.3 specifically recognizes endothelial cells (EC) in mouse blood vessels.
- Expression of a PECAM-1/CD31-related antigen was observed in a single cell type with non-differentiated morphology in early postimplantation embryos.
- The number of these cells increased progressively in early development and decreased in mature stages.
- EC in later embryonic stages (9.5, 14.5, 19.5 days postcoitum) were also recognized by mAb MEC 13.3.
Conclusions:
- The study identified an early endothelial cell marker related to PECAM-1/CD31.
- This marker's expression pattern provides insights into mouse endothelium ontogeny.
- mAb MEC 13.3 is a valuable tool for studying vascular network development in mice.