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Related Experiment Videos

Multiple roles for hepatocyte growth factor in sympathetic neuron development

F Maina1, M C Hilton, R Andres

  • 1European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.

Neuron
|June 10, 1998
PubMed
Summary

Hepatocyte growth factor (HGF)/Met signaling is crucial for sympathetic neuroblast differentiation and survival. HGF promotes neurite outgrowth in developing sympathetic neurons, suggesting an autocrine role in nervous system development.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • The development of the sympathetic nervous system involves complex signaling pathways.
  • Hepatocyte growth factor (HGF) and its receptor tyrosine kinase Met play roles in various cellular processes.

Purpose of the Study:

  • To investigate the role of HGF/Met signaling in the development of sympathetic neuroblasts and neurons.
  • To elucidate the specific functions of HGF/Met in neuroblast differentiation, proliferation, survival, and neurite outgrowth.

Main Methods:

  • In vitro studies using anti-HGF antibodies and recombinant HGF.
  • Assessment of neuroblast proliferation, differentiation, and survival.
  • Analysis of neurite outgrowth in sympathetic neurons.
  • In vivo studies utilizing a disabling mutation of the Met receptor.

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Main Results:

  • Anti-HGF antibodies decreased neuroblast differentiation into neurons and reduced neuroblast survival.
  • HGF significantly enhanced neurite outgrowth of NGF-dependent sympathetic neurons.
  • A disabling Met mutation abolished in vitro effects and increased neuroblast apoptosis in vivo.
  • Both HGF and Met are expressed in sympathetic ganglia during development.

Conclusions:

  • HGF/Met signaling is essential for sympathetic neuroblast differentiation and survival.
  • HGF promotes neurite outgrowth in developing sympathetic neurons.
  • Autocrine signaling of HGF/Met may contribute to the development of the sympathetic nervous system.