Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Embryogenesis-promoting factors in rat serum

M Katoh1, R Kimura, R Shoji

  • 1Department of Embryology, Aichi Human Service Center, Japan. miyuki@nucc.cc.nagoya-u.ac.jp

The Journal of Experimental Zoology
|June 11, 1998
PubMed
Summary

Researchers identified key proteins in rat serum that promote embryo development in vitro. These factors, including transferrin and high-density lipoprotein, are crucial for creating a defined, serum-free culture medium for whole rat embryos.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multicentric Angiographic Assessment of the Branching Patterns and Anastomotic Network of the Genicular Arteries, with Implications for Genicular Artery Embolization.

Cardiovascular and interventional radiology·2025
Same author

Frequency-domain interferometry for the determination of time delay between two extreme-ultraviolet wave packets generated by a tandem undulator.

Scientific reports·2023
Same author

Time domain double slit interference of electron produced by XUV synchrotron radiation.

Scientific reports·2023
Same author

Double-pulsed wave packets in spontaneous radiation from a tandem undulator.

Scientific reports·2022
Same author

Reply to 'Comment on "Coherent control in the extreme ultraviolet and attosecond regime by synchrotron radiation"'.

Nature communications·2021
Same author

Electron Wave Packet Interference in Atomic Inner-Shell Excitation.

Physical review letters·2021

Area of Science:

  • Developmental Biology
  • Biochemistry
  • Cell Culture Technology

Background:

  • Rat serum supports whole rat embryo culture during organogenesis.
  • A defined serum-free medium is needed for standardized whole embryo culture systems.

Purpose of the Study:

  • To isolate and identify embryogenesis-promoting factors from rat serum.
  • To lay the groundwork for a defined serum-free whole embryo culture medium.

Main Methods:

  • Rat serum fractionation using gel filtration and ion exchange chromatography.
  • Protein identification via immunoblotting and NaBr density gradient ultracentrifugation.
  • Whole rat embryo culture assays to assess growth-promoting effects.

Main Results:

Related Experiment Videos

  • Fraction B (100-500 kDa) from gel filtration supported normal embryo growth.
  • Transferrin (76 kDa), apo-AI (27 kDa), and alpha 1-inhibitor 3 (10 kDa) were identified as key embryogenesis-promoting proteins.
  • High-density lipoprotein showed a positive effect on embryogenesis, and albumin stabilized a protein fraction.

Conclusions:

  • Transferrin, high-density lipoprotein, and alpha 1-inhibitor 3 are identified as potential embryogenesis-promoting factors in rat serum.
  • Albumin may play a stabilizing or supportive role in rat embryo development in vitro.
  • These findings advance the development of defined serum-free media for whole embryo culture.