[A morphological and cytofluorometric analysis of the age structure of yeast populations]

The morphological and cytofluorometric analysis of the age structure of yeast populations of Saccharomyces cerevisiae, Candida boidinii and Candida tropicalis were carried out. The phase of cell preparation for budding and three phases of budding were accounted in morphological analysis. The latter was determined by the ratio of size of a mother cell and a bud. Cytofluorometric analysis was carried out by flow cytofluorometry of 20,000 cell which were stained by ethidium bromide. A simple method to determine the share of yeast cells in different phases of the cell cycle was proposed. The data obtained by cytofluorometry and by morphological analysis of population's age structure correlate between themselves.