Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Basic FGF-responsive telencephalic precursor cells express functional GABA(A) receptor/Cl-channels in vitro

W Ma1, Q Y Liu, D Maric

  • 1Biotechnology Research and Applications Division, Science Applications International Corporation, Rockville, Maryland 20850, USA.

Journal of Neurobiology
|June 11, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Asymmetry within social groups: division of labour and intergroup competition.

Journal of evolutionary biology·2015
Same author

Cryopreservation and Enumeration of Human Endothelial Progenitor and Endothelial Cells for Clinical Trials.

Journal of blood disorders & transfusion·2014
Same author

IRF-1 responsiveness to IFN-γ predicts different cancer immune phenotypes.

British journal of cancer·2013
Same author

Electrical breakdown in water vapor.

Physical review. E, Statistical, nonlinear, and soft matter physics·2011
Same author

Genotypic and phenotypic evaluation of Rutilus spp. from Skadar, Ohrid and Prespa Lakes supports revision of endemic as well as taxonomic status of several taxa.

Journal of fish biology·2011
Same author

Antioxidant and antimicrobial activity of some lichen species.

Mikrobiologiia·2011

Neural precursor cells in vitro express functional gamma-aminobutyric acid (GABA)A receptors, influencing cell proliferation. These receptors, composed of alpha4, beta1, and gamma1 subunits, regulate intracellular calcium levels in developing brain cells.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Previous studies identified gamma-aminobutyric acid (GABA)A receptor subunits in the cortical neuroepithelium.
  • The in vitro properties of neural precursor cells stimulated with basic fibroblast growth factor (bFGF) compared to in vivo neuroepithelial cells remain unclear.

Purpose of the Study:

  • To investigate whether neural precursor cells cultured with bFGF mimic in vivo neuroepithelial cell properties.
  • To determine the functional expression and role of GABA(A) receptors in these precursor cells.

Main Methods:

  • Neural precursor cells were isolated from embryonic rat telencephalon and cultured with bFGF.
  • Cell proliferation and differentiation were assessed.
  • Perforated patch-clamp recordings and calcium imaging were used to study GABAergic responses.

Related Experiment Videos

  • Immunohistochemistry identified GABA(A) receptor subunit expression.

    • Main Results:

    • Basic FGF-responsive cells divided and differentiated into neurons and astrocytes.
    • Cells expressed GABA(A) receptor alpha4, beta1, and gamma1 subunits.
    • GABA depolarized precursor cells and induced bicuculline-sensitive currents, indicating functional GABA(A) receptors.
    • GABA increased intracellular calcium in a bicuculline-sensitive manner.

    Conclusions:

    • Neural precursor cells cultured with bFGF exhibit functional GABA(A) receptors, suggesting these receptors are present early in development.
    • The identified GABA(A) receptor subunits (alpha4, beta1, gamma1) likely form functional chloride channels.
    • These functional GABA(A) receptors and their calcium signaling pathways may regulate neural cell proliferation in the developing brain.