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Regulation of phospholipase C-delta by GTP-binding proteins-rhoA as an inhibitory modulator
E A Hodson1, C C Ashley, A D Hughes
1Physiology Laboratory, University of Oxford, Parks Road, Oxford OX1 3PT, UK.
Biochimica Et Biophysica Acta
|June 12, 1998
Abstract:
The regulation of Phospholipase C (PLC)delta activity remains obscure. These studies show that PLCdelta1 activity is significantly enhanced by both guanosine thiotriphosphate (GTPgammaS) and Clostridium botulinum exoenzyme C3 (C3) but not by aluminium fluoride. C3 ADP ribosylated a 21-kDa protein in the PLCdelta1 preparation and Western blotting identified rhoA in these samples. RhoA acts as an inhibitory modulator of PLCdelta activity.