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Related Experiment Videos

Intrathecal bicuculline does not increase formalin-induced inflammation

J M John1, M Kaneko, D L Hammond

  • 1Department of Anesthesia and Critical Care, University of Chicago, Chicago, IL 60637, USA.

Brain Research
|June 12, 1998
PubMed
Summary

Intrathecal bicuculline, a gamma-aminobutyric acid (GABA)A receptor antagonist, enhances pain behaviors but does not increase peripheral inflammation. This suggests bicuculline acts on spinal cord nociceptive transmission, not inflammation.

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Area of Science:

  • Neuroscience
  • Pharmacology
  • Pain Research

Background:

  • Gamma-aminobutyric acid (GABA)A receptors play a role in pain modulation.
  • Bicuculline is a selective antagonist of GABA A receptors.
  • Previous studies show intrathecal bicuculline enhances pain behaviors.

Purpose of the Study:

  • To investigate if intrathecal bicuculline enhances peripheral inflammation induced by formalin.
  • To determine the mechanism by which bicuculline enhances pain behaviors.

Main Methods:

  • Formalin was injected subcutaneously into the rat hindpaw to induce inflammation and pain.
  • Plasma extravasation was measured using the Evans Blue method.
  • Rats were pretreated with intrathecal bicuculline before formalin or kaolin/carrageenan injection.

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Main Results:

  • Formalin induced a concentration-dependent increase in plasma extravasation.
  • Intrathecal bicuculline did not alter formalin-induced plasma extravasation.
  • Bicuculline also did not affect plasma extravasation induced by kaolin/carrageenan.

Conclusions:

  • The enhancement of pain behaviors by intrathecal bicuculline is not due to increased peripheral inflammation.
  • Bicuculline likely enhances nociceptive transmission in the spinal cord.
  • These findings differentiate the effects of bicuculline on pain perception versus inflammatory response.