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Expression of a 45K subunit of platelet-activating factor acetylhydrolase in the developing mouse cerebellum
H Isumi1, S Takashima, K Ikeda
1Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan.
Abstract:
The 45K subunit of platelet-activating factor acetylhydrolase (PAFAH-45K) is the product of a candidate gene for Miller-Dieker lissencephaly. We studied the expression of this protein in the developing mouse cerebellar cortex by immunochemical and immunohistochemical methods. Western blotting studies indicated that PAFAH-45K is more abundant in the fetal than the postnatal period. Immunohistochemical studies revealed developmental changes in the localization of PAFAH-45K-immunoreactivity, which shifted from the somata of Purkinje cells to the neuropil of the molecular layer. Our findings indicate that PAFAH expression is developmentally regulated and suggest its role in histogenetic processes in the cerebellar cortex other than neuronal migration.