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Age related decrease of NOR activity in bone marrow metaphase chromosomes from healthy individuals

E Pedrazzini1, N Mamaev, I Slavutsky

  • 1Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.

Molecular Pathology : MP
|June 13, 1998
PubMed
Summary
This summary is machine-generated.

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The percentage of silver-stained nucleolar organiser region (AgNOR) chromosomes in bone marrow cells decreases with age in healthy individuals. This finding in healthy bone marrow may correlate with age-related telomere shortening.

Area of Science:

  • Cytogenetics
  • Cell Biology
  • Gerontology

Background:

  • Silver-stained nucleolar organiser region (AgNOR) chromosomes are reliable markers for assessing cellular proliferation.
  • AgNORs are associated with nucleolus organizer regions (NORs) on specific chromosomes, reflecting ribosomal DNA activity.

Purpose of the Study:

  • To investigate the association between donor age and the proportion of metaphases with AgNORs in human bone marrow.
  • To determine if AgNOR counts in bone marrow cells correlate with biological aging.

Main Methods:

  • Bone marrow samples from healthy Russian and Argentinian donors were analyzed using silver staining.
  • The percentage of AgNOR-positive mitoses and AgNORs per metaphase were quantified.
  • Statistical analysis, including Kruskal Wallis test and Kendall's rank correlation, was employed to assess age-related changes.

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Main Results:

  • No significant difference in mean AgNORs per metaphase was observed between Russian and Argentinian donor groups.
  • A significant decrease in the percentage of AgNOR-positive cells was found as a function of donor age (rK = -0.57, p < 0.0012).

Conclusions:

  • The percentage of AgNOR-negative metaphases in healthy bone marrow is strongly associated with donor age.
  • This age-related decline in AgNORs may be linked to telomere shortening, a hallmark of cellular aging.