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Monocyte analysis in chronic myelomonocytic leukaemia
1Haematology Department, Fife Area Laboratory, Scotland, UK.
British Journal of Biomedical Science
|June 13, 1998
Summary
Monocytes in chronic myelomonocytic leukaemia (CMML) exhibit reduced function, impacting automated blood cell counts. Automated analysers showed varying accuracy, with Sysmex NE 8000 being least accurate for CMML monocyte enumeration.
Area of Science:
- Hematology
- Clinical Pathology
- Leukemia Research
Background:
- Monocytes in chronic myelomonocytic leukaemia (CMML) and chronic myeloid leukaemia (CML) may possess functional and morphological abnormalities.
- These abnormalities are hypothesized to lead to inaccurate counting by automated blood cell analyzers.
Purpose of the Study:
- To investigate the morphological and functional characteristics of monocytes in CMML patients.
- To evaluate the accuracy of different automated analyzers in counting CMML monocytes compared to manual methods.
Main Methods:
- Morphological analysis of monocytes from 21 normal and 14 CMML blood samples.
- Monocyte counting using manual reference methods, three automated analyzers (Sysmex NE 8000, Coulter STK-S, Sysmex SE 9000), and alpha-naphthyl acetate esterase staining.
Main Results:
- No significant morphological differences were observed between control and CMML monocytes.
- CMML monocytes showed a 40% reduction in alpha-naphthyl acetate esterase scores, indicating decreased function.
- The Sysmex NE 8000 demonstrated the lowest accuracy for CMML monocyte counts, while Coulter STK-S and Sysmex SE 9000 provided closer results to manual counts.
Conclusions:
- CMML monocytes exhibit functional deficits, evidenced by reduced esterase activity.
- Automated hematology analyzers vary in their ability to accurately count monocytes in CMML, necessitating careful selection and validation.