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Related Experiment Videos

Stable capillary coating with successive multiple ionic polymer layers

H Katayama1, Y Ishihama, N Asakawa

  • 1Department of Analytical Chemistry, Analytical Research Laboratories, Eisai Co., Ltd., Ibaraki, Japan. h1-katayama@eisai.co.jp

Analytical Chemistry
|June 13, 1998
PubMed
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A novel successive multiple ionic-polymer layer (SMIL) coating provides a stable, long-lasting modification for fused silica capillaries. This method ensures reproducible capillary performance and enables versatile applications in separations and pKa determinations.

Area of Science:

  • Analytical Chemistry
  • Materials Science

Background:

  • Fused silica capillaries are widely used in separation sciences.
  • Modifications are often needed to control electroosmotic flow (EOF) and improve reproducibility.
  • Existing methods can lack stability or tolerance to harsh conditions.

Purpose of the Study:

  • To develop a stable and reproducible capillary coating method.
  • To investigate the performance of the modified capillary for various analytical applications.
  • To achieve pH-independent electroosmotic flow (EOF) for enhanced separation capabilities.

Main Methods:

  • Developed a successive multiple ionic-polymer layer (SMIL) coating procedure.
  • Utilized dextran sulfate (DS) as the anionic polymer and Polybrene as the cationic polymer.

Related Experiment Videos

  • Tested the SMIL-DS capillary's stability, reproducibility, and tolerance to solvents and reagents.

    • Main Results:

    • Achieved a stable, long-lasting capillary coating with <1% capillary-to-capillary reproducibility.
    • Demonstrated a pH-independent EOF from pH 2-11, enabling separations under diverse conditions.
    • Successfully applied the SMIL-DS capillary for acidic protein analysis, amino acid separations, cresol isomer separations, and pKa determination.

    Conclusions:

    • The SMIL coating offers a robust and versatile modification for fused silica capillaries.
    • The SMIL-DS capillary exhibits excellent performance and broad applicability in capillary electrophoresis and chromatography.
    • This method provides a stable platform for advanced analytical separations and chemical characterization.