Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mouse model of angiogenesis

T Couffinhal1, M Silver, L P Zheng

  • 1Department of Medicine (Cardiology), St. Elizabeth's Medical Center, Tufts University School of Medicine, Boston Massachusetts 02135, USA.

The American Journal of Pathology
|June 17, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Revision of Schatzker type Ⅵ tibial plateau fracture failure focus on the recovery of lower limb alignment].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2018
Same author

Association of prenatal exposure to gestational diabetes with offspring body composition and regional body fat distribution.

Clinical obesity·2017
Same author

A robust sebum, oil, and particulate pollution model for assessing cleansing efficacy of human skin.

International journal of cosmetic science·2016
Same author

Are we measuring what we intend to measure? Implications for the management of healthcare-acquired infections.

The Journal of hospital infection·2015
Same author

[Erratum to: Mild therapeutic hypothermia].

Medizinische Klinik, Intensivmedizin und Notfallmedizin·2015
Same author

Persistently increased expression of the transforming growth factor-β1 gene in human vascular restenosis: Analysis of 62 patients with one or more episode of restenosis.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2015

Vascular endothelial growth factor (VEGF) drives therapeutic angiogenesis in ischemic muscle. This study confirms VEGF

Area of Science:

  • Cardiovascular Biology
  • Regenerative Medicine
  • Molecular Biology

Background:

  • Therapeutic angiogenesis aims to restore blood flow to ischemic tissues.
  • Vascular endothelial growth factor (VEGF) is a key regulator of blood vessel formation.
  • Endogenous VEGF's role in ischemic neovascularization requires further investigation.

Purpose of the Study:

  • To investigate the role of endogenous VEGF in postnatal collateral vessel development in ischemic hindlimb tissue.
  • To establish and characterize a murine model of hindlimb ischemia for studying angiogenesis.

Main Methods:

  • Developed a mouse model by ligating and excising the femoral artery.
  • Utilized Laser Doppler Perfusion Imaging (LDPI) to assess blood flow recovery.
  • Performed morphometric analysis of capillary density and endothelial cell proliferation (bromodeoxyuridine staining).

Related Experiment Videos

  • Quantified VEGF mRNA and protein expression.
  • Administered a neutralizing VEGF antibody to assess dependency.

    • Main Results:

    • Hindlimb ischemia led to reduced blood flow, followed by progressive recovery over 28 days.
    • Neovascularization, evidenced by increased capillary density and endothelial cell proliferation, temporally correlated with blood flow restoration.
    • VEGF mRNA and protein expression were upregulated in the ischemic hindlimb.
    • Administration of a VEGF neutralizing antibody impaired neovascularization, confirming VEGF's essential role.

    Conclusions:

    • VEGF acts as an endogenous regulator of angiogenesis in ischemic tissues.
    • The developed murine model effectively demonstrates VEGF-dependent therapeutic angiogenesis in vivo.
    • This model provides a platform for studying gene targeting effects on nutrient angiogenesis.