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Cyclin D1 and Cyclin E expression in malignant thyroid cells and in human thyroid carcinomas
D Lazzereschi1, L Sambuco, C Carnovale Scalzo
1Dipartimento di Medicina Sperimentale e Patologia, Università "La Sapienza," Rome, Italy.
International Journal of Cancer
|June 17, 1998
Summary
Altered cyclin D1 expression in the nucleus, but not cyclin E, is linked to thyroid papillary carcinomas. This suggests nuclear cyclin D1 may contribute to thyroid tumor development.
Area of Science:
- Molecular Biology
- Oncology
- Cell Cycle Regulation
Background:
- Cyclin gene alterations are increasingly implicated in human cancers.
- Understanding cyclin D1 and cyclin E expression is crucial for cancer research.
Purpose of the Study:
- To investigate the expression patterns of cyclin D1 and cyclin E in normal and malignant thyroid cells.
- To determine the role of these cyclins in thyroid tumorigenesis.
Main Methods:
- Analysis of gene expression in rat thyroid cells (normal and K-ras-transformed).
- Southern and Northern blot analyses of human thyroid tumors.
- Immunohistochemical analysis of cyclin D1 and cyclin E in thyroid tissues.
Main Results:
- K-ras-transformed cells showed elevated cyclin D1 and E compared to normal cells.
- No major genetic alterations or amplifications of cyclin D1/E genes were found in human tumors.
- Increased cyclin D1 nuclear expression was observed in 31% of papillary thyroid carcinomas.
Conclusions:
- Elevated nuclear cyclin D1 expression distinguishes a subset of thyroid papillary carcinomas.
- Nuclear cyclin D1 may play a role in the development of thyroid tumors.