Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electron microscopical study of the Fleisher ring

T Iwamoto, A G DeVoe

    Archives of Ophthalmology (Chicago, Ill. : 1960)
    |September 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Personal opinions on IDU and management of herpetic keratitis in its three different stages: epithelial, non-ulcerative stromal (metaherpetic) and chronic, ulcerative stromal.

    Highlights of ophthalmology·2014
    Same author

    Experiences with the surgery of the anophthalmic orbit.

    American journal of ophthalmology·2010
    Same author

    Repair of orbital deformities with glass wool.

    Archives of ophthalmology (Chicago, Ill. : 1929)·2010
    Same author

    Plastic visual test plates.

    American journal of ophthalmology·2010
    Same author

    Change in colonic motility after extrinsic autonomic denervation in dogs.

    Digestive diseases and sciences·1997
    Same author

    Calmodulin-binding autoinhibitory domain controls "pH-sensing" in the Na+/H+ exchanger NHE1 through sequence-specific interaction.

    Biochemistry·1997

    The Fleischer ring in keratoconus involves ferritin particle accumulation in corneal epithelium. These iron deposits were observed in intercellular spaces and vacuoles, offering insights into keratoconus pathogenesis.

    Area of Science:

    • Ophthalmology
    • Electron Microscopy
    • Corneal Diseases

    Background:

    • The Fleischer ring is a characteristic sign observed in keratoconus, an ectatic corneal disorder.
    • The precise composition and origin of the Fleischer ring have been areas of ongoing research.
    • Understanding the ultrastructural basis of the Fleischer ring may provide insights into keratoconus pathology.

    Purpose of the Study:

    • To investigate the ultrastructural characteristics of the Fleischer ring in keratoconus using transmission electron microscopy.
    • To identify the location and nature of ferritin particle accumulations within the cornea.
    • To compare findings in keratoconus with normal human corneal and conjunctival tissues.

    Main Methods:

    • Transmission electron microscopy (TEM) was employed to examine four corneal buttons from patients with keratoconus.

    Related Experiment Videos

  • Normal human corneas and conjunctivas were also analyzed using TEM for comparative purposes.
  • Detailed ultrastructural analysis focused on identifying and localizing ferritin particles.

    • Main Results:

    • Accumulations of ferritin particles were identified in widened intercellular spaces and cytoplasmic vacuoles of the corneal epithelium in keratoconus.
    • These ferritin deposits were predominantly found in the basal epithelial layers, with some observed in wing cell layers.
    • Ferritin particles were also noted scattered over the corneal epithelium, in basal conjunctival cells, and within macrophages in the conjunctival stroma, but not in corneal stroma or endothelium.

    Conclusions:

    • The Fleischer ring in keratoconus is characterized by the deposition of ferritin particles within the corneal epithelium.
    • The presence of ferritin in various corneal and conjunctival cell types suggests a complex deposition process.
    • Further research is warranted to elucidate the exact origin and pathogenic role of these ferritin deposits in keratoconus.