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Increased synovial endothelium binding and transendothelial migration of mononuclear cells during Salmonella

J Kirveskari1, S Jalkanen, O Mäki-Ikola

  • 1National Public Health Institute, Turku, Finland.

Arthritis and Rheumatism
|June 17, 1998
PubMed
Abstract

Insights

Salmonella infection increases peripheral blood mononuclear cells (PBMC) adhesion to blood vessels. These cells, carrying bacterial antigens, then migrate through vessel walls, potentially initiating arthritis.

Area of Science:

  • Immunology
  • Infectious Diseases
  • Pathophysiology

Background:

  • Salmonella infections can lead to systemic complications.
  • The role of immune cell migration in Salmonella pathogenesis is not fully understood.

Purpose of the Study:

  • To investigate the adhesion and extravasation of peripheral blood mononuclear cells (PBMC) during Salmonella infection.
  • To determine the transport of bacterial antigens within PBMC.

Main Methods:

  • Studied 13 patients with Salmonella enteritidis infection.
  • Assessed PBMC adhesion to inflamed synovial endothelium using a frozen-section assay.
  • Quantified transendothelial migration of mononuclear cells containing Salmonella antigens via immunofluorescence staining.

Main Results:

  • PBMC adhesion to synovial vessels significantly increased during Salmonella infection (P=0.0003).
  • Monocytes exhibited a heightened adhesive state 2-5 weeks post-infection.
  • Salmonella antigens were concentrated in the transmigrated PBMC population.

Conclusions:

  • Increased PBMC binding to inflamed synovial endothelium and enhanced transmigration of Salmonella-containing PBMC are key factors.
  • This process facilitates bacterial antigen transport across the endothelial barrier.
  • These mechanisms may initiate arthritis in susceptible individuals during acute Salmonella infection.

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