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Atrial natriuretic peptide increases urodilatin in the circulation
D L Vesely1, R M Overton, M Blankenship
1Department of Medicine, James A. Haley Veterans Hospital, and University of South Florida Health Sciences Center, Tampa 33612, USA.
Background:
Urodilatin is a 32-amino-acid (AA) peptide formed in the kidney.
Methods:
High-performance gel permeation chromatography and high-pressure liquid chromatography evaluation of plasma followed by sensitive urodilatin and atrial natriuretic peptide (ANP) assays revealed that urodilatin does circulate distinctly from ANP.
Results:
Urodilatin circulates at very low levels (i.e 9-12 pg/ml). Infusion of ANP increased the circulating concentration of urodilatin 135-fold (p < 0.001), suggesting that some of the effects of ANP may be mediated by urodilatin while long-acting natriuretic peptide, vessel dilator, and kaliuretic peptide did not affect urodilatin in healthy humans (n = 30). Only ANP decreased the renal clearance of urodilatin (60-75%, p < 0.01). Urodilatin was metabolized into peptides smaller than 5 AAs as well as excreted intact into urine.
Conclusion:
Urodilatin circulates and is increased by ANP in humans.