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Related Experiment Videos

Solid-phase assay for luteinizing hormone in mouse plasma

A A Gidley-Baird, B M Bindon

    Australian Journal of Biological Sciences
    |March 1, 1976
    PubMed
    Summary

    This study details a new assay for measuring luteinizing hormone (LH) in mouse plasma. The assay is sensitive and specific, enabling accurate LH level monitoring during mouse pregnancy.

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    Area of Science:

    • Endocrinology
    • Reproductive Biology
    • Assay Development

    Background:

    • Accurate measurement of luteinizing hormone (LH) is crucial for understanding reproductive physiology.
    • Existing methods may lack the sensitivity or specificity required for precise mouse plasma LH quantification.

    Purpose of the Study:

    • To develop and validate a sensitive solid-phase tube assay for quantifying mouse plasma LH levels.
    • To assess the assay's specificity and its utility in monitoring LH dynamics during mouse reproduction.

    Main Methods:

    • Development of a solid-phase tube assay using ovine LH standards and antiserum.
    • Validation of the assay by testing serial dilutions of various mouse plasma and pituitary samples.
    • Evaluation of cross-reactivity with other pituitary hormones like FSH, prolactin, and GH.
    • Measurement of plasma LH levels in hypophysectomized mice and during different stages of mouse pregnancy.

    Main Results:

    • The assay demonstrates high sensitivity ( < 0.6 ng/ml) and specificity for mouse LH.
    • Parallelism was observed with ovine LH standards in different mouse plasma matrices.
    • Minimal cross-reactivity was found with other tested hormones, except for a 12% cross-reaction with ovine TSH.
    • Significant fluctuations in plasma LH levels were detected throughout mouse pregnancy, with notable peaks before implantation and mid-pregnancy.

    Conclusions:

    • A reliable and sensitive solid-phase tube assay for mouse LH measurement has been established.
    • This assay is suitable for studying LH dynamics in various physiological and experimental conditions in mice.
    • The assay provides valuable insights into the hormonal changes governing mouse reproductive cycles and pregnancy.

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