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Evidence for oxidatively modified lipid-protein complexes in endometrium and endometriosis
A A Murphy1, W Palinski, S Rankin
1Department of Gynecology and Obstetrics, Emory University, Atlanta, Georgia 30322, USA.
Objective:
To evaluate for the presence of oxidatively modified lipid-protein complexes in endometriosis and endometrium of women with endometriosis and controls.
Design:
Controlled clinical study.
Setting:
Academic tertiary care center.
Patient(S):
Women undergoing surgery for pelvic pain, infertility, endometriosis, or tubal ligation controls.
Intervention(S):
Biopsy of endometrium and endometriosis.
Main Outcome Measure(S):
Staining with antibodies to oxidatively modified lipid proteins (HNE-7, MDA2), macrophages (HAM-56), and muscle cell actin (HHF-35).
Result(S):
Both endometrium and endometriosis tissues contain stromal cells that immunostain with HAM-56 and show immunostaining (both intracellular and extracellular) with HNE-7 and MDA2. Some endometriotic implants show patchy staining with HHF-35. Endometrium was devoid of staining with HHF-35. Control staining with nonimmune sera in both tissues was also devoid of staining.
Conclusion(S):
These data strongly implicate the occurrence of oxidative stress in endometriosis tissue. These data also suggest that oxidative modification is a normal physiological process in endometrium.