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Long-range translational coupling in single-stranded RNA bacteriophages: an evolutionary analysis

N Licis1, J van Duin, Z Balklava

  • 1Biomedical Research and Study Centre, University of Latvia, Ratsupites 1, LV1067 Riga, Latvia. licis@biomed.lu.lv

Nucleic Acids Research
|June 17, 1998
PubMed
Summary

Coliphage MS2 RNA uses a long-distance interaction (LDI) to regulate replicase synthesis. Mutations disrupting this interaction led to evolutionary adaptations, highlighting translational control

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Area of Science:

  • Molecular Biology
  • Virology
  • Genetics

Background:

  • Coliphage MS2 RNA utilizes a long-distance interaction (LDI) to repress replicase gene translation.
  • This repression is relieved by elongating ribosomes during coat gene translation, activating the replicase initiation site.
  • The Min Jou (MJ) interaction, a base pairing between nucleotides 1427-1433 and 1738-1744, underlies this translational coupling.

Purpose of the Study:

  • To investigate the biological significance of the Min Jou (MJ) interaction in controlling replicase gene translation in coliphage MS2.
  • To understand the evolutionary adaptation mechanisms following disruption of the LDI.

Main Methods:

  • Disruption of the LDI through targeted mutations in the 3'-region of the coat gene.
  • Monitoring evolutionary adaptation via phage passaging.

Related Experiment Videos

  • Analysis of pseudorevertants to identify compensatory mutations.

    • Main Results:

    • Two types of pseudorevertants were identified: those restoring the MJ interaction and those with compensatory mutations downstream.
    • Second-site mutations stabilized an adjacent LDI, restoring coat-replicase translational coupling.
    • The structure repressing the replicase start site extends to elements bordering the MJ interaction.

    Conclusions:

    • Translational control is crucial for the fitness of coliphage MS2.
    • Evolutionary pathways can restore gene regulation through direct restoration or compensatory mutations affecting RNA structure.
    • The findings elucidate the intricate RNA structural elements involved in gene regulation within bacteriophages.