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Enhanced hybridization labeling signals in Southern blotted DNAs fractionated with voltage gradient gel
1Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy.
Electrophoresis
|June 18, 1998
Abstract:
An enhancement of hybridization labeling signals is demonstrated in Southern blotted DNAs, fractionated by voltage gradient gel electrophoresis. This enhancement is due to a reduced thickness of each single nucleic acid band in the gel as a consequence of the gradient effect, corresponding to an increased concentration of DNA per unit area.