Induction of IGF-1 mRNA expression following traumatic injury to the postnatal brain

A variety of adult, non-neural tissues respond to injury by increasing expression of the gene which encodes for insulin-like growth factor-1 (IGF-1). This response is thought to be a key component in the regenerative capacity of these tissues. In contrast, the central nervous system (CNS) has relatively little regenerative capacity following injury. Interestingly, compared to many non-neuronal tissues, little IGF-1 mRNA can be detected in the adult CNS, raising the possibility that its lack of regenerative capacity is related its relative lack of IGF-1 expression. However, in the 2-week-old adolescent CNS IGF-1 mRNA can be detected in numerous brain regions. Therefore, the purpose of this study was to determine the responsiveness of the IGF-1 gene to injury in adolescent CNS tissue, a period in which expression of this gene is relatively abundant. Expression of IGF-1 mRNA was measured by means of a sensitive solution hybridization/RNase protection assay in the parieto-occipital lobes of 2-week-old and adult mice following penetrating injury. Levels of IGF-1 transcript in the injured brains were significantly increased above those of controls in both 2-week-old and adult brains 3-day post injury and remained elevated for 1 week after injury. These observations demonstrate that the adult CNS, like other tissues, can respond to injury by increasing expression of IGF-1 mRNA.