Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Highly conserved amino-acid sequence between murine STAT3 and a revised human STAT3 sequence

L D Pietra1, A Bressan, A R Pezzotti

  • 1Drug Discovery Department, Istituto di Ricerca Cesare Serono, 22 Via di Valle Caia, 00040 Ardea, Rome, Italy.

Gene
|June 19, 1998
PubMed
Summary

A revised human STAT3 sequence shows high evolutionary conservation, differing slightly from the mouse sequence. This new variant is predominantly expressed across human tissues and genomic DNA.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Statistics Measurement of Collins and Sivers Asymmetries for Transversely Polarized Deuterons.

Physical review letters·2024
Same author

Final COMPASS Results on the Transverse-Spin-Dependent Azimuthal Asymmetries in the Pion-Induced Drell-Yan Process.

Physical review letters·2024
Same author

Triangle Singularity as the Origin of the a_{1}(1420).

Physical review letters·2021
Same author

In vitro activity of fosfomycin against mucoid and non-mucoid Pseudomonas aeruginosa strains.

Journal of global antimicrobial resistance·2020
Same author

Risk factors including the presence of inflammation at the resection margins for colorectal anastomotic stenosis following surgery for diverticular disease.

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2018
Same author

First Measurement of Transverse-Spin-Dependent Azimuthal Asymmetries in the Drell-Yan Process.

Physical review letters·2017

Area of Science:

  • Molecular Biology
  • Genetics
  • Evolutionary Biology

Background:

  • Signal transducer and activator of transcription 3 (STAT3) is a key mediator in cytokine signaling pathways.
  • Previous characterization of STAT3 cDNA and protein sequences was established.

Purpose of the Study:

  • To sequence the whole human STAT3 cDNA from HepG2 cells.
  • To identify and characterize variations in the human STAT3 sequence.
  • To determine the evolutionary conservation and expression patterns of STAT3 variants.

Main Methods:

  • Whole cDNA sequencing of human STAT3 from HepG2 cells.
  • Reverse transcription-polymerase chain reaction (RT-PCR) to differentiate STAT3 sequences.
  • RT-PCR amplification of STAT3 src-homology domain 2 (SH2) from various human tissues and genomic DNA.

Related Experiment Videos

Main Results:

  • A new human STAT3 sequence was identified with 43 nucleotide and six amino-acid changes compared to the previously reported sequence.
  • The revised human STAT3 amino-acid sequence is nearly identical to the mouse sequence, indicating high evolutionary conservation.
  • Only the new SH2 variant of STAT3 was detected in 11 different human tissues and from human genomic DNA, suggesting it is the predominant form.
  • The new sequence represents a revised human STAT3 sequence or a predominant allele.

Conclusions:

  • The newly determined human STAT3 sequence is highly conserved across species.
  • The new STAT3 variant, particularly within the SH2 domain, is the predominant form expressed in human tissues and present in genomic DNA.
  • This finding suggests a revision of the canonical human STAT3 sequence or the identification of a major allele.