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Mechanism of NADH transfer among dehydrogenases
W M Arias1, H Pettersson, G Pettersson
1Department of Biochemistry, Chemical Center, University of Lund, Lund, Sweden.
Biochimica Et Biophysica Acta
|June 19, 1998
Abstract:
Steady-state and transient-state kinetic experiments have been performed to test the proposal that there is a direct (channelled) transfer of NADH from one dehydrogenase to another if the two enzymes exhibit distinct chiral coenzyme specificity (A-side vs. B-side). The results lend no support to this proposal, but are fully consistent with a free-diffusion mechanism of NADH transfer irrespective of the chiral specificity of the enzymes.