Action of the allelochemical, fischerellin A, on photosystem II

The cyanobacterium, Fischerella muscicola, produces a secondary metabolite named fischerellin A (FS) that strongly inhibits the growth of cyanobacteria and other photosynthetic organisms. The compound exhibits a unique structure and is composed of two cyclic amines and a C15 substituent that contains a double bond in the (Z) configuration and two triple bonds [L. Hagmann, F. Jüttner, Tetrahedron Lett., 37 (1996) 6539-6542]. The site of FS action is located in photosystem II (PSII). The chlorophyll fluorescence induction transient and O2 evolution methods have been used to determine the site of action of FS in PSII. FS affects the fluorescence transients, as well as O2 evolution by the cyanobacterium, Anabaena P9. The green alga, Chlamydomonas reinhardtii, and higher plants were also affected by FS in a concentration- and time-dependent fashion. FS acts at several sites which appear with increasing half-time of interaction in the following sequence: (1) effect on the rate constant of QA- reoxidation; (2) primary photochemistry trapping; (3) inactivation of PSII reaction center; and (4) segregation of individual units from grouped units. FS does not affect the photosynthetic activity of purple bacteria, Rhodospirillum rubrum.