Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A miniaturized monolayer trough with variable surface area in the square-millimeter range

C Duschl1, M Boncheva, H Vogel

  • 1Laboratoire de chimie physique des polymères et membranes, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

Biochimica Et Biophysica Acta
|June 19, 1998
PubMed
Summary

A novel miniaturized monolayer trough enables controlled compression and observation of molecular distribution using minimal substance quantities. This tool facilitates biological reactions and protein crystallization studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Temperature-induced molecular transport through polymer multilayers coated with PNIPAM microgels.

Physical chemistry chemical physics : PCCP·2015
Same author

Stability and cell uptake of calcium carbonate templated insulin microparticles.

Acta biomaterialia·2013
Same author

Filtration at the microfluidic level: enrichment of nanoparticles by tunable filters.

Journal of physics. Condensed matter : an Institute of Physics journal·2011
Same author

IR-light triggered drug delivery from micron-sized polymer biocoatings.

Journal of controlled release : official journal of the Controlled Release Society·2011
Same author

Experimental limits on weak annihilation contributions to decays.

Physical review letters·2006
Same author

New measurements of Cabibbo-suppressed decays of mesons with the CLEO-c detector.

Physical review letters·2006

Area of Science:

  • Biophysics
  • Materials Science
  • Biochemistry

Background:

  • Monolayer studies are crucial for understanding surface phenomena and molecular interactions.
  • Existing techniques often require larger sample volumes and specialized equipment.

Purpose of the Study:

  • To introduce a new, miniaturized monolayer trough for advanced surface studies.
  • To demonstrate the utility of the trough for biological assays and protein crystallization.

Main Methods:

  • Design and implementation of a miniaturized monolayer trough with controlled surface area reduction.
  • Utilizing fluorescently labeled molecules for simultaneous observation of lateral distribution during compression.
  • Conducting a biological reaction between an antibody and a lipid-anchored peptide.

Related Experiment Videos

Main Results:

  • The miniaturized trough allows for precise control over monolayer area, reducing it by a factor of two from an initial 150 mm2.
  • Demonstrated feasibility of observing molecular distribution during compression.
  • Successful execution of a specific antibody-lipid anchored peptide reaction using picomole quantities.

Conclusions:

  • The developed monolayer trough is a versatile tool for studying molecular interactions and biological processes at interfaces.
  • Its small sample requirement makes it ideal for high-throughput screening and studies with precious biomolecules.
  • Potential applications include 2D protein crystallization via specific binding sites.