Cloning and sequencing of human thrombopoietin cDNA

Human thrombopoietin (hTPO), a ligand for the protooncogene c-mpl, is the major humoral regulator of megakaryocytopoiesis and platelet production affecting the proliferation and maturation of committed cells. Recently the hTPO gene and cDNA were cloned from human genome and cDNA libraries. In this report, hTPO cDNA was synthesized by RT-PCR from a Chinese human fetal liver. The sequence of this cDNA showed a high homology with hTPO cDNA in the Genebank database (accession no. L36052) except that base substitution occurred at four sites (497, 595, 767, and 795 bp), which led to the change of three amino acid residues in the predicted protein.