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Related Experiment Videos

Filamentous phage structure, infection and assembly

D A Marvin1

  • 1Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, UK. d.a.marvin@bioc.cam.ac.uk

Current Opinion in Structural Biology
|June 19, 1998
PubMed
Summary

Structural studies of filamentous phage reveal high-resolution details of its receptor-binding domain and DNA-binding protein. This information supports models of phage infection and assembly, integrating genetic and biochemical data.

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Area of Science:

  • Structural biology
  • Virology
  • Molecular genetics

Background:

  • Filamentous phages are important model systems for studying virus-host interactions.
  • Understanding phage structure is crucial for elucidating infection mechanisms.

Purpose of the Study:

  • To determine the high-resolution structures of key filamentous phage components.
  • To integrate structural data with genetic and biochemical analyses to model virus life cycle.

Main Methods:

  • X-ray fiber diffraction for overall phage structure.
  • High-resolution structural determination of specific domains and proteins.
  • Genetic and biochemical analyses of the virus life cycle.

Main Results:

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  • A validated structural model of filamentous phage.
  • High-resolution structures of the receptor-binding domain and intracellular DNA-binding protein.
  • Dissection of the virus life cycle informed by structural data.

    • Conclusions:

    • Structural insights provide a foundation for understanding filamentous phage infection.
    • Integrated data support mechanistic models for virus assembly and propagation.