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Long-term effects of amyloid enhancing factor: clinical and experimental implications
S Shtrasburg1, M Pras, N Brezniak
1Heller Institute of Medical Research, Sheba Medical Center, Tel Hashomer, Israel.
Objective:
To study the long-term effects of amyloid enhancing factor (AEF).
Methods:
AEF, prepared from pre-amyloidotic mouse spleens, was injected intravenously into Swiss and ICR mice. This was followed by 3 daily subcutaneous injections of AgNO3, given at increasing time intervals from the administration of AEF. The mice were sacrificed on day 6 from the first AgNO3 injection, and the mean grade of amyloid deposition in the spleens was estimated using the crush and smear technique.
Results:
AEF was found to have a prolonged enhancing effect, retaining its activity for more than 3 months after its administration in both mouse strains.
Conclusions:
Experimental animals may develop amyloidosis long time after exposure to AEF. This finding may underlie the acute reoccurrence of amyloidosis sometimes observed in patients and mice long after its resolution.